• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using hnRNP K (Phospho-Ser284) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 3T3 cells using Phospho-hnRNP K (S284) Polyclonal Antibody

Anti-Phospho-HNRNPK-Ser284 antibody (250-299 aa) (STJ90527)

SKU:
STJ90527

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Heterogeneous nuclear ribonucleoprotein K-Ser284 (250-299 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPK
Gene ID: 3190
Uniprot ID: HNRPK_HUMAN
Immunogen Region: 250-299 aa
Specificity: Phospho-hnRNP K (S284) Polyclonal Antibody detects endogenous levels of hnRNP K protein only when phosphorylated at S284.
Immunogen: The antiserum was produced against synthesized peptide derived from the human hnRNP K around the phosphorylation site of Ser284 at the amino acid range 250-299
Function One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation.
Protein Name Heterogeneous Nuclear Ribonucleoprotein K
Hnrnp K
Transformation Up-Regulated Nuclear Protein
Tunp
Database Links Reactome: R-HSA-4570464
Reactome: R-HSA-72163
Reactome: R-HSA-72203
Reactome: R-HSA-9610379
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Cell Projection
Podosome
Recruited To P53/Tp53-Responsive Promoters
In The Presence Of Functional P53/Tp53
In Case Of Asfv Infection
There Is A Shift In The Localization Which Becomes Predominantly Nuclear
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoprotein K antibody
Anti-Hnrnp K antibody
Anti-Transformation Up-Regulated Nuclear Protein antibody
Anti-Tunp antibody
Anti-HNRNPK antibody
Anti-HNRPK antibody

Information sourced from Uniprot.org

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