| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Phospho-Heterogeneous nuclear ribonucleoprotein K-Ser284 (250-299 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HNRNPK |
| Gene ID: | 3190 |
| Uniprot ID: | HNRPK_HUMAN |
| Immunogen Region: | 250-299 aa |
| Specificity: | Phospho-hnRNP K (S284) Polyclonal Antibody detects endogenous levels of hnRNP K protein only when phosphorylated at S284. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human hnRNP K around the phosphorylation site of Ser284 at the amino acid range 250-299 |
| Function | One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation. |
| Protein Name | Heterogeneous Nuclear Ribonucleoprotein K Hnrnp K Transformation Up-Regulated Nuclear Protein Tunp |
| Database Links | Reactome: R-HSA-4570464 Reactome: R-HSA-72163 Reactome: R-HSA-72203 Reactome: R-HSA-9610379 |
| Cellular Localisation | Cytoplasm Nucleus Nucleoplasm Cell Projection Podosome Recruited To P53/Tp53-Responsive Promoters In The Presence Of Functional P53/Tp53 In Case Of Asfv Infection There Is A Shift In The Localization Which Becomes Predominantly Nuclear |
| Alternative Antibody Names | Anti-Heterogeneous Nuclear Ribonucleoprotein K antibody Anti-Hnrnp K antibody Anti-Transformation Up-Regulated Nuclear Protein antibody Anti-Tunp antibody Anti-HNRNPK antibody Anti-HNRPK antibody |
Information sourced from Uniprot.org
