• Western blot analysis of lysates from 293 cells treated with H2O2 100uM 15', using hnRNP C1/2 (Phospho-Ser260) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using hnRNP C1/2 (Phospho-Ser260) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of 293 cells using Phospho-hnRNP C1/2 (S260) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-HNRNPC-Ser260 antibody (231-280 aa) (STJ90910)

SKU:
STJ90910

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Heterogeneous nuclear ribonucleoproteins C1/C2-Ser260 (231-280 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPC
Gene ID: 3183
Uniprot ID: HNRPC_HUMAN
Immunogen Region: 231-280 aa
Specificity: Phospho-hnRNP C1/2 (S260) Polyclonal Antibody detects endogenous levels of hnRNP C1/2 protein only when phosphorylated at S260.
Immunogen: The antiserum was produced against synthesized peptide derived from the human hnRNP C1/2 around the phosphorylation site of Ser260 at the amino acid range 231-280
Function Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing.
Protein Name Heterogeneous Nuclear Ribonucleoproteins C1/C2
Hnrnp C1/C2
Database Links Reactome: R-HSA-4570464
Reactome: R-HSA-72163
Reactome: R-HSA-72203
Reactome: R-HSA-9013418
Reactome: R-HSA-9013422
Cellular Localisation Nucleus
Component Of Ribonucleosomes
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoproteins C1/C2 antibody
Anti-Hnrnp C1/C2 antibody
Anti-HNRNPC antibody
Anti-HNRPC antibody

Information sourced from Uniprot.org

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