• Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using HNF4 alpha (Phospho-Ser313) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of LOVO cells, using HNF4 alpha (Phospho-Ser313) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using HNF4 alpha (Phospho-Ser313) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-HNF4-Alpha (S313) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-HNF4A-Ser313 antibody (280-329 aa) (STJ91111)

SKU:
STJ91111

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Hepatocyte nuclear factor 4-alpha-Ser313 (280-329 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNF4A
Gene ID: 3172
Uniprot ID: HNF4A_HUMAN
Immunogen Region: 280-329 aa
Specificity: Phospho-HNF4-Alpha (S313) Polyclonal Antibody detects endogenous levels of HNF4-Alpha protein only when phosphorylated at S313.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HNF4 alpha around the phosphorylation site of Ser313 at the amino acid range 280-329
Function Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.
Protein Name Hepatocyte Nuclear Factor 4-Alpha
Hnf-4-Alpha
Nuclear Receptor Subfamily 2 Group A Member 1
Transcription Factor 14
Tcf-14
Transcription Factor Hnf-4
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-383280
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hepatocyte Nuclear Factor 4-Alpha antibody
Anti-Hnf-4-Alpha antibody
Anti-Nuclear Receptor Subfamily 2 Group A Member 1 antibody
Anti-Transcription Factor 14 antibody
Anti-Tcf-14 antibody
Anti-Transcription Factor Hnf-4 antibody
Anti-HNF4A antibody
Anti-HNF4 antibody
Anti-NR2A1 antibody
Anti-TCF14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance