• Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T11 antibody (STJ22169) at 1:1000 dilution. HeLa cells, NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-T11 Rabbit polyclonal antibody (STJ22169) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 polyclonal antibody (STJ22169) at 1:1000 dilution or Histone H3 antibody (A15741). HeLa, NIH/3T3 and C6 cellls were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-T11 Rabbit polyclonal antibody (STJ22169) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-Histone H3-T11 antibody (STJ22169) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using Phospho-Histone H3-T11 Rabbit polyclonal antibody (STJ22169) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat testis using Phospho-Histone H3-T11 antibody (STJ22169) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-Histone H3-T11 in paraffin-embedded mouse testis using Phospho-Histone H3-T11 Rabbit polyclonal antibody (STJ22169) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H3-T11 antibody (STJ22169) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-Histone H3-T11 in paraffin-embedded rat testis using Phospho-Histone H3-T11 Rabbit polyclonal antibody (STJ22169) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using Phospho-Histone H3-T11 rabbit polyclonal antibody (STJ22169) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Phospho-Histone H3-T11 in paraffin-embedded human lung cancer using Phospho-Histone H3-T11 Rabbit polyclonal antibody (STJ22169) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-T11 rabbit polyclonal antibody (STJ22169) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from C6 cells using Phospho-Histone H3-T11 Rabbit polyclonal antibody (STJ22169) at 1:2000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 60s.
  • Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-T11 rabbit polyclonal antibody (STJ22169) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Phospho-Histone H3-T11 antibody (STJ22169)

SKU:
STJ22169

£129.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H3-4
H3C1.H3C2.H3C3.H3C4.H3C6.
Gene ID: 8290
8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
Uniprot ID: H31T_HUMAN
H31_HUMAN
Specificity: A synthetic phosphorylated peptide around T11 of human HIST1H3B (NP_003520.1).
Immunogen Sequence: KSTGG
Tissue Specificity
Alternative Antibody Names Anti-Histone H3.1t antibody
Anti-H3/T antibody
Anti-H3t antibody
Anti-H3/G antibody
Anti-Histone H3.4 antibody
Anti-H3-4 antibody
Anti-H3FT antibody
Anti-HIST3H3 antibody
Anti-Histone H3.1 antibody
Anti-Histone H3/A antibody
Anti-Histone H3/B antibody
Anti-Histone H3/C antibody
Anti-Histone H3/D antibody
Anti-Histone H3/F antibody
Anti-Histone H3/H antibody
Anti-Histone H3/I antibody
Anti-Histone H3/J antibody
Anti-Histone H3/K antibody
Anti-Histone H3/L antibody
Anti-H3C1 antibody
Anti-H3FA antibody
Anti-HIST1H3A.H3C2 antibody
Anti-H3FL antibody
Anti-HIST1H3B.H3C3 antibody
Anti-H3FC antibody
Anti-HIST1H3C.H3C4 antibody
Anti-H3FB antibody
Anti-HIST1H3D.H3C6 antibody
Anti-H3FD antibody
Anti-HIST1H3E.H3C7 antibody
Anti-H3FI antibody
Anti-HIST1H3F.H3C8 antibody
Anti-H3FH antibody
Anti-HIST1H3G.H3C10 antibody
Anti-H3FK antibody
Anti-HIST1H3H.H3C11 antibody
Anti-H3FF antibody
Anti-HIST1H3I.H3C12 antibody
Anti-H3FJ antibody
Anti-HIST1H3J antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance