Anti-Phospho-HGS-Tyr334 antibody (301-350 aa) (STJ91095)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human HRS around the phosphorylation site of Tyr334 at the amino acid range 301-350
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Hepatocyte growth factor-regulated tyrosine kinase substrate-Tyr334 (301-350 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HGS |
| Gene ID | 9146 |
| Uniprot ID | HGS_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human HRS around the phosphorylation site of Tyr334 at the amino acid range 301-350 |
| Immunogen Region | 301-350 aa |
| Specificity | Phospho-Hrs (Y334) Polyclonal Antibody detects endogenous levels of Hrs protein only when phosphorylated at Y334. |
Additional Info
| Post Translational Modifications | Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected. Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF. Ubiquitinated. Ubiquitinated by ITCH. |
| Function | Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation. |
| Protein Name | Hepatocyte Growth Factor-Regulated Tyrosine Kinase SubstrateHrsProtein Pp110 |
| Database Links | Reactome: R-HSA-182971Reactome: R-HSA-432720Reactome: R-HSA-5689880Reactome: R-HSA-6807004Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8875360Reactome: R-HSA-9013420Reactome: R-HSA-917729Reactome: R-HSA-9635644Reactome: R-HSA-9636383Reactome: R-HSA-9706019 |
| Cellular Localisation | CytoplasmEarly Endosome MembranePeripheral Membrane ProteinCytoplasmic SideEndosomeMultivesicular Body MembraneColocalizes With Ubqln1 In Ubiquitin-Rich Cytoplasmic Aggregates That Are Not Endocytic Compartments |
| Alternative Antibody Names | Anti-Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate antibodyAnti-Hrs antibodyAnti-Protein Pp110 antibodyAnti-HGS antibodyAnti-HRS antibody |
Information sourced from Uniprot.org