• Western blot analysis of lysates from Jurkat cells, using HDAC5 (Phospho-Ser498) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of VEC cells using Phospho-HDAC5 (S498) Polyclonal Antibody diluted at 1：500
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HDAC5 (Phospho-Ser498) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-HDAC5 (S498) Polyclonal Antibody diluted at 1：500

Anti-Phospho-HDAC5-Ser498 antibody (464-513 aa) (STJ90286)

SKU:
STJ90286

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Histone deacetylase 5-Ser498 (464-513 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC5
Gene ID: 10014
Uniprot ID: HDAC5_HUMAN
Immunogen Region: 464-513 aa
Specificity: Phospho-HDAC5 (S498) Polyclonal Antibody detects endogenous levels of HDAC5 protein only when phosphorylated at S498.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HDAC5 around the phosphorylation site of Ser498 at the amino acid range 464-513
Post Translational Modifications Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.
Protein Name Histone Deacetylase 5
Hd5
Antigen Ny-Co-9
Database Links Reactome: R-HSA-2122947
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-350054
Reactome: R-HSA-8943724
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And The Cytoplasm
In Muscle Cells
It Shuttles Into The Cytoplasm During Myocyte Differentiation
The Export To Cytoplasm Depends On The Interaction With A 14-3-3 Chaperone Protein And Is Due To Its Phosphorylation At Ser-259 And Ser-498 By Ampk
Camk1 And Sik1
Alternative Antibody Names Anti-Histone Deacetylase 5 antibody
Anti-Hd5 antibody
Anti-Antigen Ny-Co-9 antibody
Anti-HDAC5 antibody
Anti-KIAA0600 antibody

Information sourced from Uniprot.org

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