Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-H2AX-Thr120 antibody (STJ197460)

Name: Anti-Phospho-H2AX-Thr120 antibody (STJ197460)
SKU: STJ197460
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

ClonalityPolyclonal

HostRabbit

ConjugationUnconjugated

IsotypeIgG

ImmunogenSynthetic Peptide of Histone H2A.X (Phospho Thr120)

STJ197460

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General Information

Short Description	Rabbit polyclonal anti-Phospho-Histone H2AX-Thr120 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications	WB
Host	Rabbit
Reactivity	Human/Mouse/Rat
Note	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Clonality	Polyclonal
Isotype	IgG
Conjugation	Unconjugated
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Dilution Range	WB 1:1000-2000
Formulation	Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Storage Instruction	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene Symbol	H2AX
Gene ID	3014
Uniprot ID	H2AX_HUMAN
Immunogen	Synthetic Peptide of Histone H2A.X (Phospho Thr120)
Specificity	The antibody detects endogenous Histone H2A.X (Phospho Thr120) protein.

Additional Info

Function	Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Protein Name	Histone H2ax H2a/X Histone H2a.x
Database Links	Reactome: R-HSA-110328 Reactome: R-HSA-110329 Reactome: R-HSA-110330 Reactome: R-HSA-110331 Reactome: R-HSA-1221632 Reactome: R-HSA-171306 Reactome: R-HSA-1912408 Reactome: R-HSA-201722 Reactome: R-HSA-212300 Reactome: R-HSA-2299718 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2559586 Reactome: R-HSA-3214858 Reactome: R-HSA-427359 Reactome: R-HSA-427389 Reactome: R-HSA-427413 Reactome: R-HSA-5250924 Reactome: R-HSA-5334118 Reactome: R-HSA-5578749 Reactome: R-HSA-5617472 Reactome: R-HSA-5625886 Reactome: R-HSA-5693565 Reactome: R-HSA-5693571 Reactome: R-HSA-5693607 Reactome: R-HSA-606279 Reactome: R-HSA-68616 Reactome: R-HSA-69473 Reactome: R-HSA-73728 Reactome: R-HSA-73772 Reactome: R-HSA-8936459 Reactome: R-HSA-8939236 Reactome: R-HSA-9018519 Reactome: R-HSA-912446 Reactome: R-HSA-9616222 Reactome: R-HSA-9670095 Reactome: R-HSA-9710421 Reactome: R-HSA-977225 Reactome: R-HSA-9821002 Reactome: R-HSA-9821993 Reactome: R-HSA-9841922 Reactome: R-HSA-9843940 Reactome: R-HSA-9843970 Reactome: R-HSA-9845323
Cellular Localisation	Nucleus Chromosome
Alternative Antibody Names	Anti-Histone H2ax antibody Anti-H2a/X antibody Anti-Histone H2a.x antibody Anti-H2AX antibody Anti-H2AFX antibody

Information sourced from Uniprot.org

Citations

View product citations for antibody STJ197460 on CiteAb