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Anti-Phospho-H2AX-Ser139 antibody [2A9-B5] (STJ99251)

Western blot analysis of extracts from untreated or Hydroxyurea-treated Hela and A549 cells, using Histone H2A.X (Phospho-Ser139) mouse mAb (1:1000 diluted) (upper) or Beta-Actin Mouse mAb (200068-8F10) (lower).Predicted band size:15KDa.Observed band size:15KDa.
Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X
Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549 (upper, untreated or Hydroxyurea-treated) and Hela (lower, untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).
Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

Anti-Phospho-H2AX-Ser139 antibody [2A9-B5] (STJ99251)

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STJ99251

£144.00 - £348.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Mouse
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Phospho-Histone H2AX-Ser139 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	2A9-B5
Conjugation:	Unconjugated
Isotype:	IgG2a
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:2000 IHC-P 1:100-500 ICC 1:400 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	H2AX
Gene ID:	3014
Uniprot ID:	H2AX_HUMAN
Specificity:	This antibody detects endogenous levels of H2A.X only when phosphorylated at serine 139.
Immunogen:	Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.

Function	Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Protein Name	Histone H2ax H2a/X Histone H2a.x
Database Links	Reactome: R-HSA-110328 Reactome: R-HSA-110329 Reactome: R-HSA-110330 Reactome: R-HSA-110331 Reactome: R-HSA-1221632 Reactome: R-HSA-171306 Reactome: R-HSA-1912408 Reactome: R-HSA-201722 Reactome: R-HSA-212300 Reactome: R-HSA-2299718 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2559586 Reactome: R-HSA-3214858 Reactome: R-HSA-427359 Reactome: R-HSA-427389 Reactome: R-HSA-427413 Reactome: R-HSA-5250924 Reactome: R-HSA-5334118 Reactome: R-HSA-5578749 Reactome: R-HSA-5617472 Reactome: R-HSA-5625886 Reactome: R-HSA-5693565 Reactome: R-HSA-5693571 Reactome: R-HSA-5693607 Reactome: R-HSA-606279 Reactome: R-HSA-68616 Reactome: R-HSA-69473 Reactome: R-HSA-73728 Reactome: R-HSA-73772 Reactome: R-HSA-8936459 Reactome: R-HSA-8939236 Reactome: R-HSA-9018519 Reactome: R-HSA-912446 Reactome: R-HSA-9616222 Reactome: R-HSA-9670095 Reactome: R-HSA-9710421 Reactome: R-HSA-977225 Reactome: R-HSA-9821002 Reactome: R-HSA-9821993 Reactome: R-HSA-9843940 Reactome: R-HSA-9843970 Reactome: R-HSA-9845323
Cellular Localisation	Nucleus Chromosome
Alternative Antibody Names	Anti-Histone H2ax antibody Anti-H2a/X antibody Anti-Histone H2a.x antibody Anti-H2AX antibody Anti-H2AFX antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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