• Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-GRK2-Tyr86 antibody (STJ193211)

SKU:
STJ193211

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Beta-adrenergic receptor kinase 1-Tyr86 is suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
WB 1:500-2000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRK2
Gene ID: 156
Uniprot ID: ARBK1_HUMAN
Specificity: This antibody detects endogenous phospho levels of GRK2 (Phospho-Tyr86) at Human:Y86, Mouse:Y86, Rat:Y86
Immunogen: Synthesized peptide derived from the human GRK2 (Phospho-Tyr86)
Function Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity. Inhibits relaxation of airway smooth muscle in response to blue light.
Protein Name Beta-Adrenergic Receptor Kinase 1
Beta-Ark-1
G-Protein Coupled Receptor Kinase 2
Database Links Reactome: R-HSA-111933
Reactome: R-HSA-416476
Reactome: R-HSA-418555
Reactome: R-HSA-5635838
Reactome: R-HSA-8856825
Cellular Localisation Cytoplasm
Cell Membrane
Postsynapse
Presynapse
Alternative Antibody Names Anti-Beta-Adrenergic Receptor Kinase 1 antibody
Anti-Beta-Ark-1 antibody
Anti-G-Protein Coupled Receptor Kinase 2 antibody
Anti-GRK2 antibody
Anti-ADRBK1 antibody
Anti-BARK antibody
Anti-BARK1 antibody

Information sourced from Uniprot.org

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