| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GRIN2B |
| Gene ID: | 2904 |
| Uniprot ID: | NMDE2_HUMAN |
| Specificity: | A synthetic phosphorylated peptide around Y1474 of human GRIN2B (NP_000825.2). |
| Immunogen Sequence: | HVYEK |
| Tissue Specificity | Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. |
| Post Translational Modifications | Phosphorylated on tyrosine residues. Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity. |
| Function | Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents. Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium. Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. |
| Protein Name | Glutamate Receptor Ionotropic - Nmda 2b Glun2b Glutamate Nmda Receptor Subunit Epsilon-2 N-Methyl D-Aspartate Receptor Subtype 2b Nmdar2b Nr2b N-Methyl-D-Aspartate Receptor Subunit 3 Nr3 Hnr3 |
| Database Links | Reactome: R-HSA-3928662 Reactome: R-HSA-438066 Reactome: R-HSA-442982 Reactome: R-HSA-5673001 Reactome: R-HSA-6794361 Reactome: R-HSA-8849932 Reactome: R-HSA-9022699 Reactome: R-HSA-9032500 Reactome: R-HSA-9609736 Reactome: R-HSA-9617324 Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Postsynaptic Cell Membrane Cell Projection Dendrite Late Endosome Lysosome Cytoplasm Cytoskeleton Co-Localizes With The Motor Protein Kif17 Along Microtubules |
| Alternative Antibody Names | Anti-Glutamate Receptor Ionotropic - Nmda 2b antibody Anti-Glun2b antibody Anti-Glutamate Nmda Receptor Subunit Epsilon-2 antibody Anti-N-Methyl D-Aspartate Receptor Subtype 2b antibody Anti-Nmdar2b antibody Anti-Nr2b antibody Anti-N-Methyl-D-Aspartate Receptor Subunit 3 antibody Anti-Nr3 antibody Anti-Hnr3 antibody Anti-GRIN2B antibody Anti-NMDAR2B antibody |
Information sourced from Uniprot.org