| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Growth factor receptor-bound protein 10-Tyr67 (33-82 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GRB10 |
| Gene ID: |
2887 |
| Uniprot ID: |
GRB10_HUMAN |
| Immunogen Region: |
33-82 aa |
| Specificity: |
Phospho-GRB10 (Y67) Polyclonal Antibody detects endogenous levels of GRB10 protein only when phosphorylated at Y67. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human GRB10 around the phosphorylation site of Tyr67 at the amino acid range 33-82 |
| Post Translational Modifications | Phosphorylated on serine residues upon EGF, FGF and PDGF stimulation. Phosphorylated at Tyr-67 by TEC. |
| Function | Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2. |
| Protein Name | Growth Factor Receptor-Bound Protein 10Grb10 Adapter ProteinInsulin Receptor-Binding Protein Grb-Ir |
| Database Links | Reactome: R-HSA-1433557Reactome: R-HSA-74713Reactome: R-HSA-74749Reactome: R-HSA-74751Reactome: R-HSA-8853659Reactome: R-HSA-9607240Reactome: R-HSA-9648895 |
| Cellular Localisation | CytoplasmWhen Complexed With Nedd4 And Igf1rFollows Igf1r InternalizationRemaining Associated With Early EndosomesUncouples From Igf1r-Containing Endosomes Before The Sorting Of The Receptor To The Lysosomal Compartment |
| Alternative Antibody Names | Anti-Growth Factor Receptor-Bound Protein 10 antibodyAnti-Grb10 Adapter Protein antibodyAnti-Insulin Receptor-Binding Protein Grb-Ir antibodyAnti-GRB10 antibodyAnti-GRBIR antibodyAnti-KIAA0207 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
