• Western blot analysis of lysates from 293 cells, using GATA4 (Phospho-Ser262) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HUVEC cells, using GATA4 (Phospho-Ser262) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GATA4-Ser262 antibody (228-277 aa) (STJ90714)

SKU:
STJ90714

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Transcription factor GATA-4-Ser262 (228-277 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATA4
Gene ID: 2626
Uniprot ID: GATA4_HUMAN
Immunogen Region: 228-277 aa
Specificity: Phospho-GATA-4 (S262) Polyclonal Antibody detects endogenous levels of GATA-4 protein only when phosphorylated at S262.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GATA4 around the phosphorylation site of Ser262 at the amino acid range 228-277
Function Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase.
Protein Name Transcription Factor Gata-4
Gata-Binding Factor 4
Database Links Reactome: R-HSA-2032785
Reactome: R-HSA-400511
Reactome: R-HSA-5578768
Reactome: R-HSA-9690406
Reactome: R-HSA-9733709
Reactome: R-HSA-9758920
Reactome: R-HSA-9823730
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Gata-4 antibody
Anti-Gata-Binding Factor 4 antibody
Anti-GATA4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance