| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-FoxO1-S256 is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FOXO1 |
| Gene ID: |
2308 |
| Uniprot ID: |
FOXO1_HUMAN |
| Immunogen: |
A synthetic phosphorylated peptide around S256 of human FoxO1 (NP_002006.2). |
| Immunogen Sequence: |
AASMD |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylation by NLK promotes nuclear export and inhibits the transcriptional activity. In response to growth factors, phosphorylation on Thr-24, Ser-256 and Ser-322 by PKB/AKT1 promotes nuclear export and inactivation of transactivational activity. Phosphorylation on Thr-24 is required for binding 14-3-3 proteins. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24 and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CDK1, leading to nuclear exclusion and loss of function. Stress signals, such as response to oxygen or nitric oxide, attenuate the PKB/AKT1-mediated phosphorylation leading to nuclear retention. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Dephosphorylated on Thr-24 and Ser-256 by PP2A in beta-cells under oxidative stress leading to nuclear retention. Phosphorylation of Ser-249 by CDK1 disrupts binding of 14-3-3 proteins leading to nuclear accumulation and has no effect on DNA-binding nor transcriptional activity. Phosphorylation by STK4/MST1 on Ser-212, upon oxidative stress, inhibits binding to 14-3-3 proteins and nuclear export. PPIA/CYPA promotes its dephosphorylation on Ser-256. Ubiquitinated by SKP2. Ubiquitination leads to proteasomal degradation. Methylation inhibits AKT1-mediated phosphorylation at Ser-256 and is increased by oxidative stress. Acetylated. Acetylation at Lys-262, Lys-265 and Lys-274 are necessary for autophagic cell death induction. Deacetylated by SIRT2 in response to oxidative stress or serum deprivation, thereby negatively regulating FOXO1-mediated autophagic cell death. Once in the nucleus, acetylated by CREBBP/EP300. Acetylation diminishes the interaction with target DNA and attenuates the transcriptional activity. It increases the phosphorylation at Ser-256. Deacetylation by SIRT1 results in reactivation of the transcriptional activity. Oxidative stress by hydrogen peroxide treatment appears to promote deacetylation and uncoupling of insulin-induced phosphorylation. By contrast, resveratrol acts independently of acetylation. Acetylated at Lys-423, promoting its localization to the nucleus and transcription factor activity. Deacetylation at Lys-423 by SIRT6, promotes its translocation into the cytoplasm, preventing its transcription factor activity. Deacetylation and subsequent inhibition by SIRT6 has different effects depending on cell types: it inhibits gluconeogenesis in hepatocytes, promotes glucose sensing in pancreatic beta-cells and regulates lipid catabolism in brown adipocytes. |
| Function | Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TTG/ATTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TTG/ATTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. Acts as an inhibitor of glucose sensing in pancreatic beta cells by acting as a transcription repressor and suppressing expression of PDX1. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC1 and PCK1. Also promotes gluconeogenesis by directly promoting expression of PPARGC1A and G6PC1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling. Regulates endothelial cell (EC) viability and apoptosis in a PPIA/CYPA-dependent manner via transcription of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. |
| Protein Name | Forkhead Box Protein O1Forkhead Box Protein O1aForkhead In Rhabdomyosarcoma |
| Database Links | Reactome: R-HSA-198693Reactome: R-HSA-210745Reactome: R-HSA-211163Reactome: R-HSA-5674400Reactome: R-HSA-5687128Reactome: R-HSA-6785807Reactome: R-HSA-9614399Reactome: R-HSA-9614657Reactome: R-HSA-9615017Reactome: R-HSA-9617629Reactome: R-HSA-9617828 |
| Cellular Localisation | CytoplasmNucleusShuttles Between The Cytoplasm And NucleusLargely Nuclear In Unstimulated CellsIn OsteoblastsColocalizes With Atf4 And Runx2 In The NucleusSerum Deprivation Increases Localization To The NucleusLeading To Activate Expression Of Sox9 And Subsequent ChondrogenesisInsulin-Induced Phosphorylation At Ser-256 By Pkb/Akt1 LeadsVia Stimulation Of Thr-24 PhosphorylationTo Binding Of 14-3-3 Proteins And Nuclear Export To The Cytoplasm Where It Is Degraded By The Ubiquitin-Proteasomal PathwayPhosphorylation At Ser-249 By Cdk1 Disrupts Binding Of 14-3-3 Proteins And Promotes Nuclear AccumulationPhosphorylation By Nlk Results In Nuclear ExportTranslocates To The Nucleus Upon Oxidative Stress-Induced Phosphorylation At Ser-212 By Stk4/Mst1Sgk1-Mediated Phosphorylation Also Results In Nuclear TranslocationRetained In The Nucleus Under Stress Stimuli Including Oxidative StressNutrient Deprivation Or Nitric OxideRetained In The Nucleus On MethylationPpia/Cypa Stimulates Its Nuclear AccumulationDeacetylation By Sirt6Promotes Its Translocation Into The Cytoplasm |
| Alternative Antibody Names | Anti-Forkhead Box Protein O1 antibodyAnti-Forkhead Box Protein O1a antibodyAnti-Forkhead In Rhabdomyosarcoma antibodyAnti-FOXO1 antibodyAnti-FKHR antibodyAnti-FOXO1A antibody |
Information sourced from Uniprot.org
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