• Western blot analysis of lysates from Jurkat cells treated PMA 125ng/ml 30', using Ezrin (Phospho-Thr566) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of HELA COLO using Phospho-Ezrin (T566) antibody. Antibody was diluted at 1:1000

Anti-Phospho-EZR-Thr566 antibody (534-583 aa) (STJ90266)

SKU:
STJ90266

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Ezrin-Thr566 (534-583 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EZR
Gene ID: 7430
Uniprot ID: EZRI_HUMAN
Immunogen Region: 534-583 aa
Specificity: Phospho-Ezrin (T566) Polyclonal Antibody detects endogenous levels of Ezrin protein only when phosphorylated at T566.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Ezrin around the phosphorylation site of Thr566 at the amino acid range 534-583
Post Translational Modifications Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Function Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Protein Name Ezrin
Cytovillin
Villin-2
P81
Database Links Reactome: R-HSA-373752
Reactome: R-HSA-437239
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Apical Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Microvillus Membrane
Ruffle Membrane
Cytoplasm
Cell Cortex
Cytoskeleton
Microvillus
Localization To The Apical Membrane Of Parietal Cells Depends On The Interaction With Pals1
Localizes To Cell Extensions And Peripheral Processes Of Astrocytes
Microvillar Peripheral Membrane Protein (Cytoplasmic Side)
Alternative Antibody Names Anti-Ezrin antibody
Anti-Cytovillin antibody
Anti-Villin-2 antibody
Anti-P81 antibody
Anti-EZR antibody
Anti-VIL2 antibody

Information sourced from Uniprot.org

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