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Anti-Phospho-ERK 1/2-Tyr204 antibody (170-219 aa) (STJ90261)

Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using p44/42 MAP Kinase (Phospho-Tyr204) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p44/42 MAP Kinase (Phospho-Tyr204) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of various cells using Phospho-ERK 1/2 (Y204) Polyclonal Antibody diluted at 1：2000
Western blot analysis of HeLa cells using Phospho-ERK 1/2 (Y204) Polyclonal Antibody diluted at 1：2000
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of mouse-spleen tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of mouse-spleen tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-spleen tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-spleen tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-ERK 1/2-Tyr204 antibody (170-219 aa) (STJ90261)

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STJ90261

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-Mitogen-activated protein kinase 3 and Mitogen-activated protein kinase 1-Tyr204 (170-219 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:10000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	MAPK3 MAPK1
Gene ID:	5595 5594
Uniprot ID:	MK03_HUMAN MK01_HUMAN
Immunogen Region:	170-219 aa
Specificity:	Phospho-ERK 1/2 (Y204) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at Y204.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human p44/42 MAP Kinase around the phosphorylation site of Tyr204 at the amino acid range 170-219

Function	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC).as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Protein Name	Mitogen-Activated Protein Kinase 3 Map Kinase 3 Mapk 3 Ert2 Extracellular Signal-Regulated Kinase 1 Erk-1 Insulin-Stimulated Map2 Kinase Map Kinase Isoform P44 P44-Mapk Microtubule-Associated Protein 2 Kinase P44-Erk1
Database Links	Reactome: R-HSA-110056 Reactome: R-HSA-112409 Reactome: R-HSA-1169408 Reactome: R-HSA-1295596 Reactome: R-HSA-162658 P27361-3 Reactome: R-HSA-170968 Reactome: R-HSA-198753 Reactome: R-HSA-202670 Reactome: R-HSA-2029482 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2559585 Reactome: R-HSA-2871796 Reactome: R-HSA-3371453 Reactome: R-HSA-375165 Reactome: R-HSA-444257 Reactome: R-HSA-445144 Reactome: R-HSA-450341 Reactome: R-HSA-456926 Reactome: R-HSA-5654726 Reactome: R-HSA-5654727 Reactome: R-HSA-5654732 Reactome: R-HSA-5654733 Reactome: R-HSA-5663213 Reactome: R-HSA-5668599 Reactome: R-HSA-5673001 Reactome: R-HSA-5674135 Reactome: R-HSA-5674499 Reactome: R-HSA-5675221 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-6811558 Reactome: R-HSA-73728 Reactome: R-HSA-74749 Reactome: R-HSA-879415 Reactome: R-HSA-881907 Reactome: R-HSA-8939211 Reactome: R-HSA-8940973 Reactome: R-HSA-8943724 Reactome: R-HSA-9627069 Reactome: R-HSA-9634638 Reactome: R-HSA-9635465 Reactome: R-HSA-9649948 Reactome: R-HSA-9652169 Reactome: R-HSA-9656223 Reactome: R-HSA-9664422 Reactome: R-HSA-982772
Cellular Localisation	Cytoplasm Nucleus Membrane Caveola Autophosphorylation At Thr-207 Promotes Nuclear Localization
Alternative Antibody Names	Anti-Mitogen-Activated Protein Kinase 3 antibody Anti-Map Kinase 3 antibody Anti-Mapk 3 antibody Anti-Ert2 antibody Anti-Extracellular Signal-Regulated Kinase 1 antibody Anti-Erk-1 antibody Anti-Insulin-Stimulated Map2 Kinase antibody Anti-Map Kinase Isoform P44 antibody Anti-P44-Mapk antibody Anti-Microtubule-Associated Protein 2 Kinase antibody Anti-P44-Erk1 antibody Anti-MAPK3 antibody Anti-ERK1 antibody Anti-PRKM3 antibody

Information sourced from Uniprot.org

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