• Immunofluorescence analysis of HepG2 cells, using Epo-R (Phospho-Tyr368) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from K562 cells, using Epo-R (Phospho-Tyr368) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of K562 cells using Phospho-EpoR (Y368) Polyclonal Antibody

Anti-Phospho-EPOR-Tyr368 antibody (341-390 aa) (STJ90712)

SKU:
STJ90712

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Erythropoietin receptor-Tyr368 (341-390 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPOR
Gene ID: 2057
Uniprot ID: EPOR_HUMAN
Immunogen Region: 341-390 aa
Specificity: Phospho-EpoR (Y368) Polyclonal Antibody detects endogenous levels of EpoR protein only when phosphorylated at Y368.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Epo-R around the phosphorylation site of Tyr368 at the amino acid range 341-390
Post Translational Modifications On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site. Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation. Ubiquitinated by NOSIP.appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.
Function Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
Protein Name Erythropoietin Receptor
Epo-R
Database Links Reactome: R-HSA-9006335
Reactome: R-HSA-9027276
Reactome: R-HSA-9027277
Reactome: R-HSA-9027283
Reactome: R-HSA-9027284
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Isoform Epor-S: Secreted
Secreted And Located To The Cell Surface
Alternative Antibody Names Anti-Erythropoietin Receptor antibody
Anti-Epo-R antibody
Anti-EPOR antibody

Information sourced from Uniprot.org

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