Anti-Phospho-Ephrin-B1/2/3-Tyr324 antibody (290-339 aa) (STJ90972)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324 at the amino acid range 290-339
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Ephrin-B1 and Ephrin-B2 and Ephrin-B3-Tyr324 (290-339 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EFNB2EFNB1194819471949EFNB2_HUMANEFNB1_HUMANEFNB3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324 at the amino acid range 290-339 |
| Immunogen Region | 290-339 aa |
| Specificity | Phospho-Ephrin-B1/2/3 (Y324) Polyclonal Antibody detects endogenous levels of Ephrin-B1/2/3 protein only when phosphorylated at Y324. |
Additional Info
| Post Translational Modifications | Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. Proteolytically processed. The ectodomain is cleaved, probably by a metalloprotease, to produce a membrane-tethered C-terminal fragment. This fragment is then further processed by the gamma-secretase complex to yield a soluble intracellular domain peptide which can translocate to the nucleus. The intracellular domain peptide is highly labile suggesting that it is targeted for degradation by the proteasome. |
| Function | Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells. Shows high affinity for the receptor tyrosine kinase EPHB1/ELK. Can also bind EPHB2 and EPHB3. Binds to, and induces collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons. |
| Protein Name | Ephrin-B1Efl-3Elk LigandElk-LEph-Related Receptor Tyrosine Kinase Ligand 2Lerk-2 Cleaved Into - Ephrin-B1 C-Terminal FragmentEphrin-B1 Ctf - Ephrin-B1 Intracellular DomainEphrin-B1 Icd |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928662Reactome: R-HSA-3928664Reactome: R-HSA-3928665 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane RaftMay Recruit Grip1 And Grip2 To Membrane Raft DomainsEphrin-B1 C-Terminal Fragment: Cell MembraneEphrin-B1 Intracellular Domain: NucleusColocalizes With Zhx2 In The Nucleus |
| Alternative Antibody Names | Anti-Ephrin-B1 antibodyAnti-Efl-3 antibodyAnti-Elk Ligand antibodyAnti-Elk-L antibodyAnti-Eph-Related Receptor Tyrosine Kinase Ligand 2 antibodyAnti-Lerk-2 Cleaved Into - Ephrin-B1 C-Terminal Fragment antibodyAnti-Ephrin-B1 Ctf - Ephrin-B1 Intracellular Domain antibodyAnti-Ephrin-B1 Icd antibodyAnti-EFNB1 antibodyAnti-EFL3 antibodyAnti-EPLG2 antibodyAnti-LERK2 antibody |
Information sourced from Uniprot.org