• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Elk1 (Phospho-Thr417) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ELK1-Thr417 antibody (379-428 aa) (STJ90257)

SKU:
STJ90257

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-ETS domain-containing protein Elk-1-Thr417 (379-428 aa) is suitable for use in Immunohistochemistry, Immunofluorescence, Immunoprecipitation and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
IP 2-5 ug mg/lysate
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELK1
Gene ID: 2002
Uniprot ID: ELK1_HUMAN
Immunogen Region: 379-428 aa
Specificity: Phospho-Elk-1 (T417) Polyclonal Antibody detects endogenous levels of Elk-1 protein only when phosphorylated at T417.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Elk1 around the phosphorylation site of Thr417 at the amino acid range 379-428
Post Translational Modifications Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention. On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1.
Function Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation.
Protein Name Ets Domain-Containing Protein Elk-1
Database Links Reactome: R-HSA-198753
Reactome: R-HSA-9031628
Reactome: R-HSA-9609690
Reactome: R-HSA-9634638
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ets Domain-Containing Protein Elk-1 antibody
Anti-ELK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance