Anti-Phospho-ELK1-Ser383 antibody (351-400 aa) (STJ90255)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Elk1 around the phosphorylation site of Ser383 at the amino acid range 351-400
General Information
| Short Description | Rabbit polyclonal anti-Phospho-ETS domain-containing protein Elk-1-Ser383 (351-400 aa) for use in WB, IHC, IF, IP and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IP 2-5 ug mg/lysateELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ELK1 |
| Gene ID | 2002 |
| Uniprot ID | ELK1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Elk1 around the phosphorylation site of Ser383 at the amino acid range 351-400 |
| Immunogen Region | 351-400 aa |
| Specificity | Phospho-Elk-1 (S383) Polyclonal Antibody detects endogenous levels of Elk-1 protein only when phosphorylated at S383. |
Additional Info
| Post Translational Modifications | Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention. On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1. Phosphorylated by PRP4K at Thr-417.phosphorylation activation ELK1 transcriptional activity. |
| Function | Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation. |
| Protein Name | Ets Domain-Containing Protein Elk-1 |
| Database Links | Reactome: R-HSA-198753Reactome: R-HSA-9031628Reactome: R-HSA-9609690Reactome: R-HSA-9634638 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ets Domain-Containing Protein Elk-1 antibodyAnti-ELK1 antibody |
Information sourced from Uniprot.org