| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Eukaryotic translation initiation factor 4E-binding protein 1-Thr46 (13-62 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EIF4EBP1 |
| Gene ID: | 1978 |
| Uniprot ID: | 4EBP1_HUMAN |
| Immunogen Region: | 13-62 aa |
| Specificity: | Phospho-4E-BP1 (T46) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at T46. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human 4E-BP1 around the phosphorylation site of Thr45 at the amino acid range 13-62 |
| Function | Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. |
| Protein Name | Eukaryotic Translation Initiation Factor 4e-Binding Protein 14e-Bp1Eif4e-Binding Protein 1Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1Phas-I |
| Database Links | Reactome: R-HSA-166208Reactome: R-HSA-72662 |
| Cellular Localisation | CytoplasmNucleusLocalization To The Nucleus Is Unaffected By Phosphorylation Status |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 4e-Binding Protein 1 antibodyAnti-4e-Bp1 antibodyAnti-Eif4e-Binding Protein 1 antibodyAnti-Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1 antibodyAnti-Phas-I antibodyAnti-EIF4EBP1 antibody |
Information sourced from Uniprot.org