Anti-Phospho-EIF4EBP1-Thr37 antibody (4-53 aa) (STJ90161)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human 4E-BP1 around the phosphorylation site of Thr36 at the amino acid range 4-53
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Eukaryotic translation initiation factor 4E-binding protein 1-Thr37 (4-53 aa) for use in WB, IHC, IF and ELISA in Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Fish, Guinea Pig and Cow samples. Datasheet included w |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Rabbit/Chicken/Monkey/Sheep/Fish/Guinea Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF4EBP1 |
| Gene ID | 1978 |
| Uniprot ID | 4EBP1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human 4E-BP1 around the phosphorylation site of Thr36 at the amino acid range 4-53 |
| Immunogen Region | 4-53 aa |
| Specificity | Phospho-4E-BP1 (T37) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at T37. |
Additional Info
| Function | Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. |
| Protein Name | Eukaryotic Translation Initiation Factor 4e-Binding Protein 14e-Bp1Eif4e-Binding Protein 1Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1Phas-I |
| Database Links | Reactome: R-HSA-166208Reactome: R-HSA-72662 |
| Cellular Localisation | CytoplasmNucleusLocalization To The Nucleus Is Unaffected By Phosphorylation Status |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 4e-Binding Protein 1 antibodyAnti-4e-Bp1 antibodyAnti-Eif4e-Binding Protein 1 antibodyAnti-Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1 antibodyAnti-Phas-I antibodyAnti-EIF4EBP1 antibody |
Information sourced from Uniprot.org