• Western blot analysis of NIH/3T3, using Phospho-EIF4EBP1-S65 antibody (STJ11105846) at 1:20000 dilution. NIH/3T3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of 293T, using Phospho-EIF4EBP1-S65 antibody (STJ11105846) at 1:20000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37 °C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-EIF4EBP1-S65 antibody [S5846RM] (STJ11105846)

SKU:
STJ11105846

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-Eukaryotic Translation Initiation Factor 4e-Binding Protein 1-S65 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5846RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF4EBP1
Gene ID: 1978
Uniprot ID: 4EBP1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S65 of human Phospho-EIF4EBP1-S65 (NP_004086.1).
Immunogen Sequence: RNSPV
Post Translational Modifications Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E. Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.
Function Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Protein Name Eukaryotic Translation Initiation Factor 4e-Binding Protein 1
4e-Bp1
Eif4e-Binding Protein 1
Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1
Phas-I
Database Links Reactome: R-HSA-166208
Reactome: R-HSA-72662
Cellular Localisation Cytoplasm
Nucleus
Localization To The Nucleus Is Unaffected By Phosphorylation Status
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4e-Binding Protein 1 antibody
Anti-4e-Bp1 antibody
Anti-Eif4e-Binding Protein 1 antibody
Anti-Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1 antibody
Anti-Phas-I antibody
Anti-EIF4EBP1 antibody

Information sourced from Uniprot.org

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