• Western blot analysis of lysates from NIH/3T3 cells, using eIF4B (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using eIF4B (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.
  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eIF4B (Phospho-Ser422) Antibody
  • Immunofluorescence analysis of NIH/3T3 cells, using eIF4B (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of 293 cells using Phospho-eIF4B (S422) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-EIF4B-Ser422 antibody (388-437 aa) (STJ90440)

SKU:
STJ90440

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Eukaryotic translation initiation factor 4B-Ser422 (388-437 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF4B
Gene ID: 1975
Uniprot ID: IF4B_HUMAN
Immunogen Region: 388-437 aa
Specificity: Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.
Immunogen: The antiserum was produced against synthesized peptide derived from the human eIF4B around the phosphorylation site of Ser422 at the amino acid range 388-437
Function Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Protein Name Eukaryotic Translation Initiation Factor 4b
Eif-4b
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-166208
Reactome: R-HSA-429947
Reactome: R-HSA-72649
Reactome: R-HSA-72662
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4b antibody
Anti-Eif-4b antibody
Anti-EIF4B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance