• Western blot analysis of Hela cells using Phospho-CRMP-2 (S522) Polyclonal Antibody diluted at 1:2000

Anti-Phospho-DPYSL2-Ser522 antibody (STJ90673)

SKU:
STJ90673

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Dihydropyrimidinase-related protein 2-Ser522 is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DPYSL2
Gene ID: 1808
Uniprot ID: DPYL2_HUMAN
Specificity: Phospho-CRMP-2 (S522) Polyclonal Antibody detects endogenous levels of CRMP-2 protein only when phosphorylated at S522.
Immunogen: Synthesized phospho-peptide around the phosphorylation site of human CRMP-2 (phospho Ser522)
Post Translational Modifications 3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509. Phosphorylation at Thr-514 by GSK3B abolishes tubulin-binding leading to destabilization of microtubule assembly in axons and neurodegeneration. Phosphorylation by DYRK2 at Ser-522 is required for subsequent phosphorylation by GSK3B.
Function Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.
Protein Name Dihydropyrimidinase-Related Protein 2
Drp-2
Collapsin Response Mediator Protein 2
Crmp-2
N2a3
Unc-33-Like Phosphoprotein 2
Ulip-2
Database Links Reactome: R-HSA-399956
Reactome: R-HSA-437239
Cellular Localisation Cytoplasm
Cytosol
Cytoskeleton
Membrane
Tightly But Non-Covalently Associated With Membranes
Alternative Antibody Names Anti-Dihydropyrimidinase-Related Protein 2 antibody
Anti-Drp-2 antibody
Anti-Collapsin Response Mediator Protein 2 antibody
Anti-Crmp-2 antibody
Anti-N2a3 antibody
Anti-Unc-33-Like Phosphoprotein 2 antibody
Anti-Ulip-2 antibody
Anti-DPYSL2 antibody
Anti-CRMP2 antibody
Anti-ULIP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance