• Western blot analysis of extracts of various cell lines, using Phospho-DLG2-Y340 antibody (STJ11101118) at 1:1000 dilution. 293T cells were treated by ATP (5 mM) at 30 °C for 1 hour. NIH/3T3 cells were treated by ATP (5 mM) at 30 °C for 1 hour. C6 cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using Phospho-PSD93/chapsyn-110/DLG2-Y340 Rabbit polyclonal antibody (STJ11101118) at 1:1000 dilution. 293T cells were treated by ATP (5 mM) at 30 °C for 1 hour. NIH/3T3 cells were treated by ATP (5 mM) at 30 °C for 1 hour. C6 cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-DLG2-Y340 antibody (STJ11101118)

SKU:
STJ11101118

£129.50 - £642.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DLG2
Gene ID: 1740
Uniprot ID: DLG2_HUMAN
Specificity: A synthetic phosphorylated peptide around Y340 of human PSD93/chapsyn-110/DLG2 (NP_001355.2).
Immunogen Sequence: DDYTR
Post Translational Modifications Palmitoylation of isoform 1 is not required for targeting to postsynaptic density.
Function Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses.
Protein Name Disks Large Homolog 2
Channel-Associated Protein Of Synapse-110
Chapsyn-110
Postsynaptic Density Protein Psd-93
Database Links Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-6794361
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cell Membrane
Lipid-Anchor
Postsynaptic Density
Synapse
Membrane
Cell Projection
Axon
Perikaryon
Concentrated In Soma And Postsynaptic Density Of A Subset Of Neurons
Alternative Antibody Names Anti-Disks Large Homolog 2 antibody
Anti-Channel-Associated Protein Of Synapse-110 antibody
Anti-Chapsyn-110 antibody
Anti-Postsynaptic Density Protein Psd-93 antibody
Anti-DLG2 antibody

Information sourced from Uniprot.org

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