Anti-Phospho-CTNNA1-Ser641 antibody (607-656 aa) (STJ91176)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Catenin-alpha1 around the phosphorylation site of Ser641 at the amino acid range 607-656
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Catenin alpha-1-Ser641 (607-656 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CTNNA1 |
| Gene ID | 1495 |
| Uniprot ID | CTNA1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Catenin-alpha1 around the phosphorylation site of Ser641 at the amino acid range 607-656 |
| Immunogen Region | 607-656 aa |
| Specificity | Phospho-Catenin-Alpha 1 (S641) Polyclonal Antibody detects endogenous levels of Catenin-Alpha 1 protein only when phosphorylated at S641. |
Additional Info
| Post Translational Modifications | Sumoylated. Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion. |
| Function | Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. May play a crucial role in cell differentiation. |
| Protein Name | Catenin Alpha-1Alpha E-CateninCadherin-Associated ProteinRenal Carcinoma Antigen Ny-Ren-13 |
| Database Links | Reactome: R-HSA-418990Reactome: R-HSA-5218920Reactome: R-HSA-525793Reactome: R-HSA-5626467Reactome: R-HSA-9762292Reactome: R-HSA-9764302Reactome: R-HSA-9833576 |
| Cellular Localisation | CytoplasmCytoskeletonCell JunctionAdherens JunctionCell MembranePeripheral Membrane ProteinCytoplasmic SideNucleusFound At Cell-Cell Boundaries And Probably At Cell-Matrix BoundariesIsoform 3: Cell Membrane |
| Alternative Antibody Names | Anti-Catenin Alpha-1 antibodyAnti-Alpha E-Catenin antibodyAnti-Cadherin-Associated Protein antibodyAnti-Renal Carcinoma Antigen Ny-Ren-13 antibodyAnti-CTNNA1 antibody |
Information sourced from Uniprot.org