| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Alpha-crystallin B chain-crystallin-Ser19 (10-59 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CRYAB |
| Gene ID: | 1410 |
| Uniprot ID: | CRYAB_HUMAN |
| Immunogen Region: | 10-59 aa |
| Specificity: | Phospho-Crystallin-Alpha B (S19) Polyclonal Antibody detects endogenous levels of Crystallin-Alpha B protein only when phosphorylated at S19. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human CRYAB around the phosphorylation site of Ser19 at the amino acid range 10-59 |
| Function | May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome. |
| Protein Name | Alpha-Crystallin B Chain Alpha(B-Crystallin Heat Shock Protein Beta-5 Hspb5 Heat Shock Protein Family B Member 5 Renal Carcinoma Antigen Ny-Ren-27 Rosenthal Fiber Component |
| Database Links | Reactome: R-HSA-3371571 |
| Cellular Localisation | Cytoplasm Nucleus Secreted Lysosome Translocates To The Nucleus During Heat Shock And Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles Localizes At The Z-Bands And The Intercalated Disk In Cardiomyocytes Can Be Secreted The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative Antibody Names | Anti-Alpha-Crystallin B Chain antibody Anti-Alpha(B-Crystallin antibody Anti-Heat Shock Protein Beta-5 antibody Anti-Hspb5 antibody Anti-Heat Shock Protein Family B Member 5 antibody Anti-Renal Carcinoma Antigen Ny-Ren-27 antibody Anti-Rosenthal Fiber Component antibody Anti-CRYAB antibody Anti-CRYA2 antibody Anti-HSPB5 antibody |
Information sourced from Uniprot.org