• Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using CREB (Phospho-Ser142) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CREB (Phospho-Ser142) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using CREB (Phospho-Ser142) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of HeLa cells using Phospho-CREB-1 (S142) Polyclonal Antibody diluted at 1：500
  • Western blot analysis of various cells using Phospho-CREB-1 (S142) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CREB1-Ser142 antibody (111-160 aa) (STJ91016)

SKU:
STJ91016

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Cyclic AMP-responsive element-binding protein 1-Ser142 (111-160 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CREB1
Gene ID: 1385
Uniprot ID: CREB1_HUMAN
Immunogen Region: 111-160 aa
Specificity: Phospho-CREB-1 (S142) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S142.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CREB around the phosphorylation site of Ser142 at the amino acid range 111-160
Function Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling.
Protein Name Cyclic Amp-Responsive Element-Binding Protein 1
Creb-1
Camp-Responsive Element-Binding Protein 1
Database Links Reactome: R-HSA-111931
Reactome: R-HSA-111932
Reactome: R-HSA-198693
Reactome: R-HSA-199920
Reactome: R-HSA-2151201
Reactome: R-HSA-2197563
Reactome: R-HSA-375165
Reactome: R-HSA-400253
Reactome: R-HSA-442720
Reactome: R-HSA-442729
Reactome: R-HSA-442742
Reactome: R-HSA-5674400
Reactome: R-HSA-881907
Reactome: R-HSA-9022692
Reactome: R-HSA-9022699
Reactome: R-HSA-9022702
Reactome: R-HSA-9022707
Reactome: R-HSA-9031628
Reactome: R-HSA-9609690
Reactome: R-HSA-9616222
Reactome: R-HSA-9634638
Reactome: R-HSA-9660821
Reactome: R-HSA-9664323
Reactome: R-HSA-9707616
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cyclic Amp-Responsive Element-Binding Protein 1 antibody
Anti-Creb-1 antibody
Anti-Camp-Responsive Element-Binding Protein 1 antibody
Anti-CREB1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance