| Host: |
Rabbit |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Coronin-1B-Ser-2 is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICC 1:100IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CORO1B |
| Gene ID: |
57175 |
| Uniprot ID: |
COR1B_HUMAN |
| Specificity: |
The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed |
| Immunogen: |
Coronin-1B (Ser-2) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 2 in human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B and in coronin-1A. However, this |
| Post Translational Modifications | Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location. |
| Function | Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction. |
| Protein Name | Coronin-1bCoronin-2 |
| Cellular Localisation | CytoplasmCytoskeletonStress FiberLocalized To The Leading Edge In FibroblastsAs Well As Weakly Along Actin Stress Fibers |
| Alternative Antibody Names | Anti-Coronin-1b antibodyAnti-Coronin-2 antibodyAnti-CORO1B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
