• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western Blot analysis of 1 HEK-293 cell, 2, LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CEP55-Ser425 antibody (STJ91247)

SKU:
STJ91247

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Centrosomal protein of 55 kDa-Ser425 is suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CEP55
Gene ID: 55165
Uniprot ID: CEP55_HUMAN
Specificity: Phospho-CEP55 (S425) Polyclonal Antibody detects endogenous levels of CEP55 protein only when phosphorylated at S425.
Immunogen: Synthesized phospho-peptide around the phosphorylation site of human CEP55 (phospho Ser425)
Post Translational Modifications There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.
Function Plays a role in mitotic exit and cytokinesis. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis. Not required for microtubule nucleation. Plays a role in the development of the brain and kidney.
Protein Name Centrosomal Protein Of 55 Kda
Cep55
Up-Regulated In Colon Cancer 6
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Cleavage Furrow
Midbody
Midbody Ring
Present At The Centrosomes At Interphase
A Small Portion Is Associated Preferentially With The Mother Centriole
Whereas The Majority Localizes To The Pericentriolar Material
During Mitosis
Loses Affinity For The Centrosome At The Onset Of Prophase And Diffuses Throughout The Cell
This Dissociation From The Centrosome Is Phosphorylation-Dependent
May Remain Localized At The Centrosome During Mitosis In Certain Cell Types
Appears At The Cleavage Furrow In Late Anaphase And In The Midbody In Cytokinesis
Alternative Antibody Names Anti-Centrosomal Protein Of 55 Kda antibody
Anti-Cep55 antibody
Anti-Up-Regulated In Colon Cancer 6 antibody
Anti-CEP55 antibody
Anti-C10orf3 antibody
Anti-URCC6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance