Western blot analysis of various lysates using Phospho-CDK1/2/3-T14 Rabbit mAb (STJ11102635) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37 ยฐC for 20 hours or treated by Nocodazole (100 ng/mL) at 37 ยฐC for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-CDK1/CDK2/CDK3-T14 antibody [S5MR] (STJ11102635)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102635
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General Information

Short DescriptionRabbit monoclonal Phospho-CDK1/2/3-T14 antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS5MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCDK3
CDK2
1018
1017
983
CDK3_HUMAN
CDK2_HUMAN
CDK1_HUMAN
Immunogen SequenceEGTYG
SpecificityA synthetic phosphorylated peptide around T14 of human CDK1/2/3CDK1 (P06493).

Additional Info

Tissue Specificity Q00526 - Expressed in cancer cell lines and glioblastoma tissue.
P24941 - NA
P06493 - Isoform 2: Found in breast cancer tissues.
Q00526 - Expressed in cancer cell lines and glioblastoma tissue.
P24941 - NA
P06493 - Isoform 2: Found in breast cancer tissues.
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 3 antibody
Anti-Cell Division Protein Kinase 3 antibody
Anti-CDK3 antibody
Anti-CDKN3 antibody
Anti-Cyclin-Dependent Kinase 2 antibody
Anti-Cell Division Protein Kinase 2 antibody
Anti-P33 Protein Kinase antibody
Anti-CDK2 antibody
Anti-CDKN2 antibody
Anti-Cyclin-Dependent Kinase 1 antibody
Anti-Cdk1 antibody
Anti-Cell Division Control Protein 2 Homolog antibody
Anti-Cell Division Protein Kinase 1 antibody
Anti-P34 Protein Kinase antibody
Anti-CDK1 antibody
Anti-CDC2 antibody
Anti-CDC28A antibody
Anti-CDKN1 antibody
Anti-P34CDC2 antibody
Anti-Cyclin-Dependent Kinase 3 antibody
Anti-Cell Division Protein Kinase 3 antibody
Anti-CDK3 antibody
Anti-CDKN3 antibody
Anti-Cyclin-Dependent Kinase 2 antibody
Anti-Cell Division Protein Kinase 2 antibody
Anti-P33 Protein Kinase antibody
Anti-CDK2 antibody
Anti-CDKN2 antibody
Anti-Cyclin-Dependent Kinase 1 antibody
Anti-Cdk1 antibody
Anti-Cell Division Control Protein 2 Homolog antibody
Anti-Cell Division Protein Kinase 1 antibody
Anti-P34 Protein Kinase antibody
Anti-CDK1 antibody
Anti-CDC2 antibody
Anti-CDC28A antibody
Anti-CDKN1 antibody
Anti-P34CDC2 antibody

Information sourced from Uniprot.org

Citations

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