Western blot analysis of various lysates using Phospho-CDK1/2/3-T14 Rabbit mAb (STJ11102635) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37 °C for 20 hours or treated by Nocodazole (100 ng/mL) at 37 °C for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-CDK1/CDK2/CDK3-T14 antibody [S5MR] (STJ11102635)

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STJ11102635
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Short Description :Rabbit monoclonal Phospho-CDK1/2/3-T14 antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Monoclonal
Clone ID :S5MR
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:CDK3
CDK2
1018
1017
983
CDK3_HUMAN
CDK2_HUMAN
CDK1_HUMAN
Immunogen Sequence:EGTYG
Specificity:A synthetic phosphorylated peptide around T14 of human CDK1/2/3CDK1 (P06493).
Tissue Specificity Q00526 - Expressed in cancer cell lines and glioblastoma tissue.
P24941 - NA
P06493 - Isoform 2: Found in breast cancer tissues.
Q00526 - Expressed in cancer cell lines and glioblastoma tissue.
P24941 - NA
P06493 - Isoform 2: Found in breast cancer tissues.
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 3 antibody
Anti-Cell Division Protein Kinase 3 antibody
Anti-CDK3 antibody
Anti-CDKN3 antibody
Anti-Cyclin-Dependent Kinase 2 antibody
Anti-Cell Division Protein Kinase 2 antibody
Anti-P33 Protein Kinase antibody
Anti-CDK2 antibody
Anti-CDKN2 antibody
Anti-Cyclin-Dependent Kinase 1 antibody
Anti-Cdk1 antibody
Anti-Cell Division Control Protein 2 Homolog antibody
Anti-Cell Division Protein Kinase 1 antibody
Anti-P34 Protein Kinase antibody
Anti-CDK1 antibody
Anti-CDC2 antibody
Anti-CDC28A antibody
Anti-CDKN1 antibody
Anti-P34CDC2 antibody
Anti-Cyclin-Dependent Kinase 3 antibody
Anti-Cell Division Protein Kinase 3 antibody
Anti-CDK3 antibody
Anti-CDKN3 antibody
Anti-Cyclin-Dependent Kinase 2 antibody
Anti-Cell Division Protein Kinase 2 antibody
Anti-P33 Protein Kinase antibody
Anti-CDK2 antibody
Anti-CDKN2 antibody
Anti-Cyclin-Dependent Kinase 1 antibody
Anti-Cdk1 antibody
Anti-Cell Division Control Protein 2 Homolog antibody
Anti-Cell Division Protein Kinase 1 antibody
Anti-P34 Protein Kinase antibody
Anti-CDK1 antibody
Anti-CDC2 antibody
Anti-CDC28A antibody
Anti-CDKN1 antibody
Anti-P34CDC2 antibody

Information sourced from Uniprot.org

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