• Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using VE-Cadherin (Phospho-Tyr731) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using VE-Cadherin (Phospho-Tyr731) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CDH5-Tyr731 antibody (697-746 aa) (STJ90968)

SKU:
STJ90968

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Cadherin-5-Tyr731 (697-746 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDH5
Gene ID: 1003
Uniprot ID: CADH5_HUMAN
Immunogen Region: 697-746 aa
Specificity: Phospho-VE-Cadherin (Y731) Polyclonal Antibody detects endogenous levels of VE-Cadherin protein only when phosphorylated at Y731.
Immunogen: The antiserum was produced against synthesized peptide derived from the human VE-Cadherin around the phosphorylation site of Tyr731 at the amino acid range 697-746
Post Translational Modifications Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. O-glycosylated.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.
Protein Name Cadherin-5
7b4 Antigen
Vascular Endothelial Cadherin
Ve-Cadherin
Cd Antigen Cd144
Database Links Reactome: R-HSA-418990
Reactome: R-HSA-5218920
Cellular Localisation Cell Junction
Cell Membrane
Single-Pass Type I Membrane Protein
Found At Cell-Cell Boundaries And Probably At Cell-Matrix Boundaries
Krit1 And Cdh5 Reciprocally Regulate Their Localization To Endothelial Cell-Cell Junctions
Alternative Antibody Names Anti-Cadherin-5 antibody
Anti-7b4 Antigen antibody
Anti-Vascular Endothelial Cadherin antibody
Anti-Ve-Cadherin antibody
Anti-Cd Antigen Cd144 antibody
Anti-CDH5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance