• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Cdc6-S54 rabbit monoclonal antibody (STJ11102646) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (1 uM) at 37 °C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Cdc6-S54 Rabbit monoclonal antibody (STJ11102646) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (1 uM) at 37 °C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-CDC6-S54 antibody [S6MR] (STJ11102646)

SKU:
STJ11102646

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-Cdc6-S54 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC6
Gene ID: 990
Uniprot ID: CDC6_HUMAN
Immunogen: A synthetic phosphorylated peptide around S54 of human Cdc6 (Q99741).
Immunogen Sequence: PLSPR
Post Translational Modifications Ubiquitinated by the SCF(CCNF) E3 ubiquitin-protein ligase complex.
Function Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Protein Name Cell Division Control Protein 6 Homolog
Cdc6-Related Protein
Cdc18-Related Protein
Hscdc18
P62(Cdc6
Hscdc6
Database Links Reactome: R-HSA-1362277
Reactome: R-HSA-176187
Reactome: R-HSA-68689
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-68962
Reactome: R-HSA-69017
Reactome: R-HSA-69205
Cellular Localisation Nucleus
Cytoplasm
The Protein Is Nuclear In G1 And Cytoplasmic In S-Phase Cells
Alternative Antibody Names Anti-Cell Division Control Protein 6 Homolog antibody
Anti-Cdc6-Related Protein antibody
Anti-Cdc18-Related Protein antibody
Anti-Hscdc18 antibody
Anti-P62(Cdc6 antibody
Anti-Hscdc6 antibody
Anti-CDC6 antibody
Anti-CDC18L antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance