• Western blot analysis of lysates from RAW264.7 cells treated with PMA 125ng/ml 30', using CCR5 (Phospho-Ser349) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-CKR-5 (S349) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-CCR5-Ser349 antibody (303-352 aa) (STJ90450)

SKU:
STJ90450

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-C-C chemokine receptor type 5-Ser349 (303-352 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCR5
Gene ID: 1234
Uniprot ID: CCR5_HUMAN
Immunogen Region: 303-352 aa
Specificity: Phospho-CKR-5 (S349) Polyclonal Antibody detects endogenous levels of CKR-5 protein only when phosphorylated at S349.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CCR5 around the phosphorylation site of Ser349 at the amino acid range 303-352
Post Translational Modifications Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4. O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site even if Ser-7 may be also O-glycosylated. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen. Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry. Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
Function Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor. (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1.
Protein Name C-C Chemokine Receptor Type 5
C-C Ckr-5
Cc-Ckr-5
Ccr-5
Ccr5
Chemr13
Hiv-1 Fusion Coreceptor
Cd Antigen Cd195
Database Links Reactome: R-HSA-173107
Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-6783783
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-C-C Chemokine Receptor Type 5 antibody
Anti-C-C Ckr-5 antibody
Anti-Cc-Ckr-5 antibody
Anti-Ccr-5 antibody
Anti-Ccr5 antibody
Anti-Chemr13 antibody
Anti-Hiv-1 Fusion Coreceptor antibody
Anti-Cd Antigen Cd195 antibody
Anti-CCR5 antibody
Anti-CMKBR5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance