| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-G1/S-specific cyclin-E1-Thr395 (361-410 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CCNE1 |
| Gene ID: | 898 |
| Uniprot ID: | CCNE1_HUMAN |
| Immunogen Region: | 361-410 aa |
| Specificity: | Phospho-Cyclin E1 (T395) Polyclonal Antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at T395. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Cyclin E1 around the phosphorylation site of Thr395 at the amino acid range 361-410 |
| Post Translational Modifications | Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2.appears to occur independently of phosphorylation. |
| Function | Essential for the control of the cell cycle at the G1/S (start) transition. |
| Protein Name | G1/S-Specific Cyclin-E1 |
| Database Links | Reactome: R-HSA-1538133 Reactome: R-HSA-187577 Reactome: R-HSA-2559586 Reactome: R-HSA-390471 Reactome: R-HSA-6804116 Reactome: R-HSA-69017 Reactome: R-HSA-69200 Reactome: R-HSA-69202 Reactome: R-HSA-69205 Reactome: R-HSA-69231 Reactome: R-HSA-69563 Reactome: R-HSA-8849470 Reactome: R-HSA-9661069 Reactome: R-HSA-9706019 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-G1/S-Specific Cyclin-E1 antibody Anti-CCNE1 antibody Anti-CCNE antibody |
Information sourced from Uniprot.org