• Western blot analysis of lysates from HeLa cells treated with Paclitaxel 1uM 60', using Cyclin E1 (Phospho-Thr395) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of HeLa cells using Phospho-Cyclin E1 (T395) Polyclonal Antibody diluted at 1：2000
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Cyclin E1 (Phospho-Thr395) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Cyclin E1 (T395) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-CCNE1-Thr395 antibody (361-410 aa) (STJ90241)

SKU:
STJ90241

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-G1/S-specific cyclin-E1-Thr395 (361-410 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNE1
Gene ID: 898
Uniprot ID: CCNE1_HUMAN
Immunogen Region: 361-410 aa
Specificity: Phospho-Cyclin E1 (T395) Polyclonal Antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at T395.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Cyclin E1 around the phosphorylation site of Thr395 at the amino acid range 361-410
Post Translational Modifications Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2.appears to occur independently of phosphorylation.
Function Essential for the control of the cell cycle at the G1/S (start) transition.
Protein Name G1/S-Specific Cyclin-E1
Database Links Reactome: R-HSA-1538133
Reactome: R-HSA-187577
Reactome: R-HSA-2559586
Reactome: R-HSA-390471
Reactome: R-HSA-6804116
Reactome: R-HSA-69017
Reactome: R-HSA-69200
Reactome: R-HSA-69202
Reactome: R-HSA-69205
Reactome: R-HSA-69231
Reactome: R-HSA-69563
Reactome: R-HSA-8849470
Reactome: R-HSA-9661069
Reactome: R-HSA-9706019
Cellular Localisation Nucleus
Alternative Antibody Names Anti-G1/S-Specific Cyclin-E1 antibody
Anti-CCNE1 antibody
Anti-CCNE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance