Anti-Phospho-CBL-Tyr-700 antibody [M161] (STJA0005316)

SKU:
STJA0005316-100

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Host: Mouse
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Phospho-c-Cbl-Tyr-700 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: M161
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Protein A Purified
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBL
Gene ID: 867
Uniprot ID: CBL_HUMAN
Specificity: The antibody detects a 120 kDa* protein in human Jurkat cells treated with pervanadate, but not in control cells.
Immunogen: Clone (M161) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 700 in human c-Cbl. This sequence is highly conserved in rat and mouse c-Cbl.
Post Translational Modifications Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70. Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not phosphorylated at Tyr-731 by FGFR3. Phosphorylated on tyrosine residues by activated CSF1R, PDGFRA and PDGFRB. Phosphorylated on tyrosine residues by HCK. Ubiquitinated, leading to its degradation via the proteasome. Ubiquitination is negatively regulated by IFT20.
Function Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Ubiquitinates SPRY2. Ubiquitinates EGFR. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA.
Protein Name E3 Ubiquitin-Protein Ligase Cbl
Casitas B-Lineage Lymphoma Proto-Oncogene
Proto-Oncogene C-Cbl
Ring Finger Protein 55
Ring-Type E3 Ubiquitin Transferase Cbl
Signal Transduction Protein Cbl
Database Links Reactome: R-HSA-1059683
Reactome: R-HSA-1236382
Reactome: R-HSA-1295596
Reactome: R-HSA-1433559
Reactome: R-HSA-182971
Reactome: R-HSA-2173789
Reactome: R-HSA-5637810
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-6807004
Reactome: R-HSA-8849469
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8875360
Reactome: R-HSA-912631
Reactome: R-HSA-9680350
Reactome: R-HSA-9706369
Reactome: R-HSA-9706377
Cellular Localisation Cytoplasm
Cell Membrane
Cell Projection
Cilium
Golgi Apparatus
Colocalizes With Fgfr2 In Lipid Rafts At The Cell Membrane
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Cbl antibody
Anti-Casitas B-Lineage Lymphoma Proto-Oncogene antibody
Anti-Proto-Oncogene C-Cbl antibody
Anti-Ring Finger Protein 55 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Cbl antibody
Anti-Signal Transduction Protein Cbl antibody
Anti-CBL antibody
Anti-CBL2 antibody
Anti-RNF55 antibody

Information sourced from Uniprot.org

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