| Host: |
Mouse |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Phospho-c-Cbl-Tyr-700 is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M161 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Protein A Purified |
| Dilution Range: |
WB 1:1000IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CBL |
| Gene ID: |
867 |
| Uniprot ID: |
CBL_HUMAN |
| Specificity: |
The antibody detects a 120 kDa* protein in human Jurkat cells treated with pervanadate, but not in control cells. |
| Immunogen: |
Clone (M161) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 700 in human c-Cbl. This sequence is highly conserved in rat and mouse c-Cbl. |
| Post Translational Modifications | Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70. Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not phosphorylated at Tyr-731 by FGFR3. Phosphorylated on tyrosine residues by activated CSF1R, PDGFRA and PDGFRB. Phosphorylated on tyrosine residues by HCK. Ubiquitinated, leading to its degradation via the proteasome. Ubiquitination is negatively regulated by IFT20. |
| Function | Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Ubiquitinates SPRY2. Ubiquitinates EGFR. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA. |
| Protein Name | E3 Ubiquitin-Protein Ligase CblCasitas B-Lineage Lymphoma Proto-OncogeneProto-Oncogene C-CblRing Finger Protein 55Ring-Type E3 Ubiquitin Transferase CblSignal Transduction Protein Cbl |
| Database Links | Reactome: R-HSA-1059683Reactome: R-HSA-1236382Reactome: R-HSA-1295596Reactome: R-HSA-1433559Reactome: R-HSA-182971Reactome: R-HSA-2173789Reactome: R-HSA-5637810Reactome: R-HSA-5654726Reactome: R-HSA-5654727Reactome: R-HSA-5654732Reactome: R-HSA-5654733Reactome: R-HSA-6807004Reactome: R-HSA-8849469Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8875360Reactome: R-HSA-912631Reactome: R-HSA-9680350Reactome: R-HSA-9706369Reactome: R-HSA-9706377 |
| Cellular Localisation | CytoplasmCell MembraneCell ProjectionCiliumGolgi ApparatusColocalizes With Fgfr2 In Lipid Rafts At The Cell Membrane |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Cbl antibodyAnti-Casitas B-Lineage Lymphoma Proto-Oncogene antibodyAnti-Proto-Oncogene C-Cbl antibodyAnti-Ring Finger Protein 55 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Cbl antibodyAnti-Signal Transduction Protein Cbl antibodyAnti-CBL antibodyAnti-CBL2 antibodyAnti-RNF55 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
