Anti-Phospho-CASP9-Thr125 antibody (91-140 aa) (STJ90758)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Caspase 9 around the phosphorylation site of Thr125 at the amino acid range 91-140
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Caspase-9-Thr125 (91-140 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CASP9 |
| Gene ID | 842 |
| Uniprot ID | CASP9_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Caspase 9 around the phosphorylation site of Thr125 at the amino acid range 91-140 |
| Immunogen Region | 91-140 aa |
| Specificity | Phospho-Caspase-9 (T125) Polyclonal Antibody detects endogenous levels of Caspase-9 protein only when phosphorylated at T125. |
Additional Info
| Post Translational Modifications | Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl.occurs in the response of cells to DNA damage. (Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP9 processing, preventing CASP9 activation and ability to mediate intrinsic apoptosis. Ubiquitinated by BIRC6.this activity is inhibited by DIABLO/SMAC. |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC. Isoform 2: Lacks activity is an dominant-negative inhibitor of caspase-9. |
| Protein Name | Caspase-9Casp-9Apoptotic Protease Mch-6Apoptotic Protease-Activating Factor 3Apaf-3Ice-Like Apoptotic Protease 6Ice-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10 |
| Database Links | Reactome: R-HSA-111458Reactome: R-HSA-111459Reactome: R-HSA-111463Reactome: R-HSA-111464Reactome: R-HSA-168638Reactome: R-HSA-198323Reactome: R-HSA-418889Reactome: R-HSA-5674400Reactome: R-HSA-9627069 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Caspase-9 antibodyAnti-Casp-9 antibodyAnti-Apoptotic Protease Mch-6 antibodyAnti-Apoptotic Protease-Activating Factor 3 antibodyAnti-Apaf-3 antibodyAnti-Ice-Like Apoptotic Protease 6 antibodyAnti-Ice-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10 antibodyAnti-CASP9 antibodyAnti-MCH6 antibody |
Information sourced from Uniprot.org