• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Caspase 9 (Phospho-Thr125) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 5'+calyculinA 50ng/ml 5', using Caspase 9 (Phospho-Thr125) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Caspase-9 (T125) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CASP9-Thr125 antibody (91-140 aa) (STJ90758)

SKU:
STJ90758

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Caspase-9-Thr125 (91-140 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CASP9
Gene ID: 842
Uniprot ID: CASP9_HUMAN
Immunogen Region: 91-140 aa
Specificity: Phospho-Caspase-9 (T125) Polyclonal Antibody detects endogenous levels of Caspase-9 protein only when phosphorylated at T125.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Caspase 9 around the phosphorylation site of Thr125 at the amino acid range 91-140
Post Translational Modifications Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl.occurs in the response of cells to DNA damage. (Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP9 processing, preventing CASP9 activation and ability to mediate intrinsic apoptosis. Ubiquitinated by BIRC6.this activity is inhibited by DIABLO/SMAC.
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC. Isoform 2: Lacks activity is an dominant-negative inhibitor of caspase-9.
Protein Name Caspase-9
Casp-9
Apoptotic Protease Mch-6
Apoptotic Protease-Activating Factor 3
Apaf-3
Ice-Like Apoptotic Protease 6
Ice-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10
Database Links Reactome: R-HSA-111458
Reactome: R-HSA-111459
Reactome: R-HSA-111463
Reactome: R-HSA-111464
Reactome: R-HSA-168638
Reactome: R-HSA-198323
Reactome: R-HSA-418889
Reactome: R-HSA-5674400
Reactome: R-HSA-9627069
Alternative Antibody Names Anti-Caspase-9 antibody
Anti-Casp-9 antibody
Anti-Apoptotic Protease Mch-6 antibody
Anti-Apoptotic Protease-Activating Factor 3 antibody
Anti-Apaf-3 antibody
Anti-Ice-Like Apoptotic Protease 6 antibody
Anti-Ice-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10 antibody
Anti-CASP9 antibody
Anti-MCH6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance