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Anti-Phospho-BRAF-Ser446 antibody (411-460 aa) (STJ90940)

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using B-RAF (Phospho-Ser446) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of K562 293T lysis using Phospho-Raf-B (S446) antibody. Antibody was diluted at 1:1000
Immunohistochemistry analysis of paraffin-embedded human brain, using B-RAF (Phospho-Ser446) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-BRAF-Ser446 antibody (411-460 aa) (STJ90940)

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STJ90940

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase B-raf-Ser446 (411-460 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	BRAF
Gene ID:	673
Uniprot ID:	BRAF_HUMAN
Immunogen Region:	411-460 aa
Specificity:	Phospho-Raf-B (S446) Polyclonal Antibody detects endogenous levels of Raf-B protein only when phosphorylated at S446.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human B-RAF around the phosphorylation site of Ser446 at the amino acid range 411-460

Post Translational Modifications	Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149.which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.
Function	Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons.
Protein Name	Serine/Threonine-Protein Kinase B-Raf Proto-Oncogene B-Raf P94 V-Raf Murine Sarcoma Viral Oncogene Homolog B1
Database Links	Reactome: R-HSA-1295596 Reactome: R-HSA-170968 Reactome: R-HSA-170984 Reactome: R-HSA-187706 Reactome: R-HSA-5673000 Reactome: R-HSA-5674135 Reactome: R-HSA-5674499 Reactome: R-HSA-5675221 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9726840 Reactome: R-HSA-9726842
Cellular Localisation	Nucleus Cytoplasm Cell Membrane Colocalizes With Rgs14 And Raf1 In Both The Cytoplasm And Membranes
Alternative Antibody Names	Anti-Serine/Threonine-Protein Kinase B-Raf antibody Anti-Proto-Oncogene B-Raf antibody Anti-P94 antibody Anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 antibody Anti-BRAF antibody Anti-BRAF1 antibody Anti-RAFB1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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