Anti-Phospho-BLM-Thr99 antibody (65-114 aa) (STJ91066)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Bloom Syndrome around the phosphorylation site of Thr99 at the amino acid range 65-114
General Information
| Short Description | Rabbit polyclonal anti-Phospho-RecQ-like DNA helicase BLM-Thr99 (65-114 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BLM |
| Gene ID | 641 |
| Uniprot ID | BLM_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Bloom Syndrome around the phosphorylation site of Thr99 at the amino acid range 65-114 |
| Immunogen Region | 65-114 aa |
| Specificity | Phospho-BLM (T99) Polyclonal Antibody detects endogenous levels of BLM protein only when phosphorylated at T99. |
Additional Info
| Function | ATP-dependent DNA helicase that unwinds double-stranded (ds)DNA in a 3'-5' direction. Participates in DNA replication and repair. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and Holliday junction DNA. Unwinds G-quadruplex DNA.unwinding occurs in the 3'-5' direction and requires a 3' single-stranded end of at least 7 nucleotides. Helicase activity is higher on G-quadruplex substrates than on duplex DNA substrates. Telomeres, immunoglobulin heavy chain switch regions and rDNA are notably G-rich.formation of G-quadruplex DNA would block DNA replication and transcription. Negatively regulates sister chromatid exchange (SCE). Recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks. (Microbial infection) Eliminates nuclear HIV-1 cDNA, thereby suppressing immune sensing and proviral hyper-integration. |
| Protein Name | Recq-Like Dna Helicase BlmBloom Syndrome ProteinDna 3'-5' Helicase BlmDna Helicase - Recq-Like Type 2Recq2Recq Protein-Like 3 |
| Database Links | Reactome: R-HSA-174414Reactome: R-HSA-3108214Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-912446Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusTogether With SpidrIs Redistributed In Discrete Nuclear Dna Damage-Induced Foci Following Hydroxyurea (Hu) Or Camptothecin (Cpt) TreatmentAccumulated At Sites Of Dna Damage In A Rmi Complex- And Spidr-Dependent Manner |
| Alternative Antibody Names | Anti-Recq-Like Dna Helicase Blm antibodyAnti-Bloom Syndrome Protein antibodyAnti-Dna 3'-5' Helicase Blm antibodyAnti-Dna Helicase - Recq-Like Type 2 antibodyAnti-Recq2 antibodyAnti-Recq Protein-Like 3 antibodyAnti-BLM antibodyAnti-RECQ2 antibodyAnti-RECQL3 antibody |
Information sourced from Uniprot.org