| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-RecQ-like DNA helicase BLM-Thr99 (65-114 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BLM |
| Gene ID: | 641 |
| Uniprot ID: | BLM_HUMAN |
| Immunogen Region: | 65-114 aa |
| Specificity: | Phospho-BLM (T99) Polyclonal Antibody detects endogenous levels of BLM protein only when phosphorylated at T99. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Bloom Syndrome around the phosphorylation site of Thr99 at the amino acid range 65-114 |
| Function | ATP-dependent DNA helicase that unwinds double-stranded (ds)DNA in a 3'-5' direction. Participates in DNA replication and repair. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and Holliday junction DNA. Unwinds G-quadruplex DNA.unwinding occurs in the 3'-5' direction and requires a 3' single-stranded end of at least 7 nucleotides. Helicase activity is higher on G-quadruplex substrates than on duplex DNA substrates. Telomeres, immunoglobulin heavy chain switch regions and rDNA are notably G-rich.formation of G-quadruplex DNA would block DNA replication and transcription. Negatively regulates sister chromatid exchange (SCE). Recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks. (Microbial infection) Eliminates nuclear HIV-1 cDNA, thereby suppressing immune sensing and proviral hyper-integration. |
| Protein Name | Recq-Like Dna Helicase BlmBloom Syndrome ProteinDna 3'-5' Helicase BlmDna Helicase - Recq-Like Type 2Recq2Recq Protein-Like 3 |
| Database Links | Reactome: R-HSA-174414Reactome: R-HSA-3108214Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-912446Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusTogether With SpidrIs Redistributed In Discrete Nuclear Dna Damage-Induced Foci Following Hydroxyurea (Hu) Or Camptothecin (Cpt) TreatmentAccumulated At Sites Of Dna Damage In A Rmi Complex- And Spidr-Dependent Manner |
| Alternative Antibody Names | Anti-Recq-Like Dna Helicase Blm antibodyAnti-Bloom Syndrome Protein antibodyAnti-Dna 3'-5' Helicase Blm antibodyAnti-Dna Helicase - Recq-Like Type 2 antibodyAnti-Recq2 antibodyAnti-Recq Protein-Like 3 antibodyAnti-BLM antibodyAnti-RECQ2 antibodyAnti-RECQL3 antibody |
Information sourced from Uniprot.org
