• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BLK (Phospho-Tyr501) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BLK-Tyr501 antibody (456-505 aa) (STJ91198)

SKU:
STJ91198

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein kinase Blk-Tyr501 (456-505 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BLK
Gene ID: 640
Uniprot ID: BLK_HUMAN
Immunogen Region: 456-505 aa
Specificity: Phospho-Blk (Y501) Polyclonal Antibody detects endogenous levels of Blk protein only when phosphorylated at Y501.
Immunogen: The antiserum was produced against synthesized peptide derived from the human BLK around the phosphorylation site of Tyr501 at the amino acid range 456-505
Post Translational Modifications Phosphorylated on tyrosine residues after antibody-mediated surface engagement of the B-cell antigen receptor (BCR). Ubiquitination of activated BLK by the UBE3A ubiquitin protein ligase leads to its degradation by the ubiquitin-proteasome pathway.
Function Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Phosphorylates CGAS, promoting retention of CGAS in the cytosol.
Protein Name Tyrosine-Protein Kinase Blk
B Lymphocyte Kinase
P55-Blk
Database Links Reactome: R-HSA-8939245
Reactome: R-HSA-983695
Cellular Localisation Cell Membrane
Lipid-Anchor
Present And Active In Lipid Rafts
Membrane Location Is Required For The Phosphorylation Of Cd79a And Cd79b
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Blk antibody
Anti-B Lymphocyte Kinase antibody
Anti-P55-Blk antibody
Anti-BLK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance