| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Bcl-2-like protein 1-Thr47 (13-62 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:40000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BCL2L1 |
| Gene ID: | 598 |
| Uniprot ID: | B2CL1_HUMAN |
| Immunogen Region: | 13-62 aa |
| Specificity: | Phospho-Bcl-x (T47) Polyclonal Antibody detects endogenous levels of Bcl-x protein only when phosphorylated at T47. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human BCL-XL around the phosphorylation site of Thr47 at the amino acid range 13-62 |
| Post Translational Modifications | Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis. Ubiquitinated by RNF183 during prolonged ER stress, leading to degradation by the proteosome. |
| Function | Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. Isoform Bcl-X(S) promotes apoptosis. |
| Protein Name | Bcl-2-Like Protein 1 Bcl2-L-1 Apoptosis Regulator Bcl-X |
| Database Links | Reactome: R-HSA-111453 Reactome: R-HSA-6785807 Reactome: R-HSA-844455 Reactome: R-HSA-9648002 Reactome: R-HSA-9692913 Reactome: R-HSA-9702518 Reactome: R-HSA-9818030 |
| Cellular Localisation | Isoform Bcl-X(L): Mitochondrion Inner Membrane Mitochondrion Outer Membrane Mitochondrion Matrix Cytoplasmic Vesicle Secretory Vesicle Synaptic Vesicle Membrane Cytoplasm Cytosol Cytoskeleton Microtubule Organizing Center Centrosome Nucleus Membrane Single-Pass Membrane Protein Cytoplasmic Side After Neuronal Stimulation Translocates From Cytosol To Synaptic Vesicle And Mitochondrion Membrane In A Calmodulin-Dependent Manner Localizes To The Centrosome When Phosphorylated At Ser-49 |
| Alternative Antibody Names | Anti-Bcl-2-Like Protein 1 antibody Anti-Bcl2-L-1 antibody Anti-Apoptosis Regulator Bcl-X antibody Anti-BCL2L1 antibody Anti-BCL2L antibody Anti-BCLX antibody |
Information sourced from Uniprot.org