• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BAD (Phospho-Ser112) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from 293 cells treated with Forskolin, using BAD (Phospho-Ser112) Antibody. The lane on the left is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Bad (S112) Polyclonal Antibody

Anti-Phospho-BAD-Ser112 antibody (78-127 aa) (STJ90188)

SKU:
STJ90188

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Bcl2-associated agonist of cell death-Ser112 (78-127 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAD
Gene ID: 572
Uniprot ID: BAD_HUMAN
Immunogen Region: 78-127 aa
Specificity: Phospho-Bad (S112) Polyclonal Antibody detects endogenous levels of Bad protein only when phosphorylated at S112.
Immunogen: The antiserum was produced against synthesized peptide derived from the human BAD around the phosphorylation site of Ser112 at the amino acid range 78-127
Post Translational Modifications Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis. Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.
Function Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Protein Name Bcl2-Associated Agonist Of Cell Death
Bad
Bcl-2-Binding Component 6
Bcl-2-Like Protein 8
Bcl2-L-8
Bcl-Xl/Bcl-2-Associated Death Promoter
Bcl2 Antagonist Of Cell Death
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-111453
Reactome: R-HSA-193648
Reactome: R-HSA-198323
Reactome: R-HSA-5674400
Cellular Localisation Mitochondrion Outer Membrane
Cytoplasm
Colocalizes With Hif3a In The Cytoplasm
Upon Phosphorylation
Locates To The Cytoplasm
Alternative Antibody Names Anti-Bcl2-Associated Agonist Of Cell Death antibody
Anti-Bad antibody
Anti-Bcl-2-Binding Component 6 antibody
Anti-Bcl-2-Like Protein 8 antibody
Anti-Bcl2-L-8 antibody
Anti-Bcl-Xl/Bcl-2-Associated Death Promoter antibody
Anti-Bcl2 Antagonist Of Cell Death antibody
Anti-BAD antibody
Anti-BBC6 antibody
Anti-BCL2L8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance