| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Bad-S112 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BAD |
| Gene ID: |
572 |
| Uniprot ID: |
BAD_HUMAN |
| Immunogen: |
A synthetic phosphorylated peptide around S112 of mouse Bad (NP_031548.1?). |
| Immunogen Sequence: |
HSSYP |
| Tissue Specificity | Expressed in a wide variety of tissues. |
| Post Translational Modifications | Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis. Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99. |
| Function | Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. |
| Protein Name | Bcl2-Associated Agonist Of Cell DeathBadBcl-2-Binding Component 6Bcl-2-Like Protein 8Bcl2-L-8Bcl-Xl/Bcl-2-Associated Death PromoterBcl2 Antagonist Of Cell Death |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-111453Reactome: R-HSA-193648Reactome: R-HSA-198323Reactome: R-HSA-5674400 |
| Cellular Localisation | Mitochondrion Outer MembraneCytoplasmColocalizes With Hif3a In The CytoplasmUpon PhosphorylationLocates To The Cytoplasm |
| Alternative Antibody Names | Anti-Bcl2-Associated Agonist Of Cell Death antibodyAnti-Bad antibodyAnti-Bcl-2-Binding Component 6 antibodyAnti-Bcl-2-Like Protein 8 antibodyAnti-Bcl2-L-8 antibodyAnti-Bcl-Xl/Bcl-2-Associated Death Promoter antibodyAnti-Bcl2 Antagonist Of Cell Death antibodyAnti-BAD antibodyAnti-BBC6 antibodyAnti-BCL2L8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
