• Western blot analysis of extracts of 293T cells, using Phospho-Na+/K+-ATPase-S16 antibody (STJ11101103) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from 293T cells, using Phospho-Na+/K+-ATPase-S16 Rabbit polyclonal antibody (STJ11101103) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-ATP1A1-S16 antibody (STJ11101103)

SKU:
STJ11101103

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Na+/K+-ATPase-S16 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP1A1
Gene ID: 476
Uniprot ID: AT1A1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S16 of human Na+/K+-ATPase (NP_000692.2).
Immunogen Sequence: AVSEQ
Post Translational Modifications Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity.
Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Protein Name Sodium/Potassium-Transporting Atpase Subunit Alpha-1
Na(+/K(+ Atpase Alpha-1 Subunit
Sodium Pump Subunit Alpha-1
Database Links Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Reactome: R-HSA-9679191
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Sarcolemma
Cell Projection
Axon
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Sodium/Potassium-Transporting Atpase Subunit Alpha-1 antibody
Anti-Na(+/K(+ Atpase Alpha-1 Subunit antibody
Anti-Sodium Pump Subunit Alpha-1 antibody
Anti-ATP1A1 antibody

Information sourced from Uniprot.org

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