| Host: | Rabbit |
| Applications: | WB/IHC/ICC/IP |
| Reactivity: | Human/Mouse/Rat/Bovine/Canine |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Aquaporin 2-Ser 269 is suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Purification: | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range: | WB 1:1000 IHC 1:600-1:5000 ICC 1:200-1:500 IP 3 ul |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Aqp2 |
| Gene ID: | 25386 |
| Uniprot ID: | AQP2_RAT |
| Immunogen: | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269 conjugated to KLH. |
| Tissue Specificity | Detected in kidney, in cortical and the medullary collecting tubules (at protein level). Detected in kidney medulla and cortex. |
| Post Translational Modifications | Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. N-glycosylated. |
| Function | Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis. Could also be permeable to glycerol. |
| Protein Name | Aquaporin-2 Aqp-2 Adh Water Channel Aquaporin-Cd Aqp-Cd Collecting Duct Water Channel Protein Wch-Cd Water Channel Protein For Renal Collecting Duct |
| Database Links | Reactome: R-RNO-432040 Reactome: -RNO-432047 |
| Cellular Localisation | Apical Cell Membrane Multi-Pass Membrane Protein Basolateral Cell Membrane Cell Membrane Cytoplasmic Vesicle Membrane Golgi Apparatus Trans-Golgi Network Membrane Shuttles From Vesicles To The Apical Membrane Vasopressin-Regulated Phosphorylation Is Required For Translocation To The Apical Cell Membrane Plekha8/Fapp2 Is Required To Transport Aqp2 From The Tgn To Sites Where Aqp2 Is Phosphorylated |
| Alternative Antibody Names | Anti-Aquaporin-2 antibody Anti-Aqp-2 antibody Anti-Adh Water Channel antibody Anti-Aquaporin-Cd antibody Anti-Aqp-Cd antibody Anti-Collecting Duct Water Channel Protein antibody Anti-Wch-Cd antibody Anti-Water Channel Protein For Renal Collecting Duct antibody Anti-Aqp2 antibody |
Information sourced from Uniprot.org
