• Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (No. STJA0003582, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
  • Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (No. STJA0003582, red, 1:1000) and total AQP2 (green). Nuclei labeled with DAPI. Magnification 600x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
  • Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser269 in the vasopressin (AVP) treated lane (+) , but not in the control lane (-).

Anti-Phospho-AQP2-Ser 269 antibody (STJA0003582)

SKU:
STJA0003582-100

£442.50
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Host: Rabbit
Applications: WB/IHC/ICC/IP
Reactivity: Human/Mouse/Rat/Bovine/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Aquaporin 2-Ser 269 is suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC 1:600-1:5000
ICC 1:200-1:500
IP 3 ul
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Aqp2
Gene ID: 25386
Uniprot ID: AQP2_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269 conjugated to KLH.
Tissue Specificity Detected in kidney, in cortical and the medullary collecting tubules (at protein level). Detected in kidney medulla and cortex.
Post Translational Modifications Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. N-glycosylated.
Function Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis.
Protein Name Aquaporin-2
Aqp-2
Adh Water Channel
Aquaporin-Cd
Aqp-Cd
Collecting Duct Water Channel Protein
Wch-Cd
Water Channel Protein For Renal Collecting Duct
Database Links Reactome: R-RNO-432040
Reactome: -RNO-432047
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Cell Membrane
Cytoplasmic Vesicle Membrane
Golgi Apparatus
Trans-Golgi Network Membrane
Shuttles From Vesicles To The Apical Membrane
Vasopressin-Regulated Phosphorylation Is Required For Translocation To The Apical Cell Membrane
Plekha8/Fapp2 Is Required To Transport Aqp2 From The Tgn To Sites Where Aqp2 Is Phosphorylated
Alternative Antibody Names Anti-Aquaporin-2 antibody
Anti-Aqp-2 antibody
Anti-Adh Water Channel antibody
Anti-Aquaporin-Cd antibody
Anti-Aqp-Cd antibody
Anti-Collecting Duct Water Channel Protein antibody
Anti-Wch-Cd antibody
Anti-Water Channel Protein For Renal Collecting Duct antibody
Anti-Aqp2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance