Anti-Phospho-AKT1-Thr342 antibody (STJA0003576)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic phospho-peptide corresponding to amino acid residues surrounding Thr342 conjugated to KLH.
General Information
| Short Description | Rabbit polyclonal anti-Phospho-AKT-Thr342 for use in WB in D.melanogaster samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | D.melanogaster |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range | WB 1:500 |
| Formulation | 100 ยตl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 ยตg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr342 conjugated to KLH. |
Additional Info
| Background | The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms ( Alpha, Beta, and Gamma ) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Akt Alpha occurs at two specific regulatory sites in Drosophila, one localized 342 in the kinase domain, Thr, and the other in the C-terminal 505 regulatory domain, Ser these two activation sites are 473 308 homologous to mammalian Ser and Thr respectively (Powell et al., 2004). |
Information sourced from Uniprot.org