| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
D.melanogaster |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-AKT-Thr342 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Purification: |
This antibody was antigen affinity purified from pooled serum. |
| Dilution Range: |
WB 1:500IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunogen: |
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr342 conjugated to KLH. |
| Background | The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms ( Alpha, Beta, and Gamma ) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Akt Alpha occurs at two specific regulatory sites in Drosophila, one localized 342 in the kinase domain, Thr, and the other in the C-terminal 505 regulatory domain, Ser these two activation sites are 473 308 homologous to mammalian Ser and Thr respectively (Powell et al., 2004). |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
